Journal: Cell Reports Methods

Article Title: Long-term imaging of individual mRNA molecules in living cells

doi: 10.1016/j.crmeth.2022.100226

Figure Lengend Snippet:

Article Snippet: 8×PP7 and 10×PP7 fragment were amplified from pLH-sgRNA-Sirius-8×PP7 (Addgene #121940 ( )) and CMV-10×PP7 oligo-library based (Addgene #158199 ( )) respectively and replacing 12×PP7 to create cmv-mCherry-8×PP7 and cmv-mCherry-10×PP7.

Techniques: Recombinant, Software

Journal: The Journal of Cell Biology

Article Title: Slk19p Is a Centromere Protein That Functions to Stabilize Mitotic Spindles

doi:

Figure Lengend Snippet: Genotypes and Plasmids Used in this Study

Article Snippet: The SLK19 gene was cloned by complementation with a TRP1-CEN plasmid library (created by F. Spencer and P. Hieter; Johns Hopkins Medical School, Baltimore, MD) by selection for growth on 5-FOA.

Techniques: Plasmid Preparation